Test Code LAB511 Adrenocorticotropic Hormone (ACTH), Plasma
Additional Codes
Mayo Test Code: ACTH
Reporting Name
Adrenocorticotropic Hormone, PUseful For
Determining the cause of hypercortisolism and hypocortisolism
Method Name
Electrochemiluminescence Immunoassay
Performing Laboratory

Specimen Type
Plasma EDTASpecimen Required
Patient Preparation: 12 hours before this blood test do not take multivitamins or dietary supplements containing biotin or vitamin B7, which are commonly found in hair, skin, and nail supplements and multivitamins.
Collection Container/Tube: Ice-cooled, lavender top (EDTA)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions:
1. Morning (6 a.m.-10:30 a.m.) specimen is desirable.
2. Collect with a pre-chilled EDTA tube and transport to the laboratory on ice.
3. Spin down in a refrigerated centrifuge within 2 hours and immediately separate plasma from cells.
4. Immediately freeze plasma.
Specimen Minimum Volume
0.5 mL
Specimen Stability Information
Specimen Type | Temperature | Time |
---|---|---|
Plasma EDTA | Frozen (preferred) | 28 days |
Refrigerated | 3 hours | |
Ambient | 2 hours |
Reject Due To
Hemolysis |
Mild OK; Gross reject |
Lipemia |
Mild OK; Gross OK |
Icterus |
NA |
Other |
NA |
Reference Values
7.2-63 pg/mL (a.m. draws)
No established reference values for p.m. draws
Pediatric reference values are the same as adults, as confirmed by peer reviewed literature.
Petersen KE: ACTH in normal children and children with pituitary and adrenal diseases. I. Measurement in plasma by radioimmunoassay-basal values. Acta Paediatr Scand 1981;70:341-345
For SI unit Reference Values, see https://www.mayomedicallaboratories.com/order-tests/si-unit-conversion.html.
Day(s) and Time(s) Performed
Monday through Friday; 5 a.m.-12 a.m., Saturday; 6 a.m.-6 p.m.
CPT Code Information
82024
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
ACTH | Adrenocorticotropic Hormone, P | 2141-0 |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
ACTH | Adrenocorticotropic Hormone, P | 2141-0 |
Test Classification
This test has been cleared or approved by the U.S. Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.Forms
If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:
General Request Form (T239) (http://www.mayomedicallaboratories.com/it-mmfiles/general-request-form.pdf)
Oncology Test Request Form (T729) (http://www.mayomedicallaboratories.com/it-mmfiles/oncology-request-form.pdf)