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Test Code VIRNR Viral Culture, Non-Respiratory

Reporting Name

Viral Culture, Non Respiratory

Useful For

Diagnosing viral infections in nonrespiratory specimens

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
TISSR Tissue Processing No, (Bill Only) No
VID2 Additional Testing Virus Ident No, (Bill Only) No
SVIR Viral Smear, Shell Vial No, (Bill Only) No

Testing Algorithm

All routine viral cultures are inoculated into cell culture tubes for viral detection. The most common specimens received for routine testing include body fluid, rectal, spinal fluid, and stool. A rapid (16-hour incubation) shell vial cell culture assay will be inoculated when specimens are designated for herpes simplex virus (HSV) or cytomegalovirus (CMV) detection, or as appropriate for source indicated.

 

Blood, lymph node tissue, and bone marrow/bone tissue specimens are not good sources for viral culture and are frequently toxic to cell culture lines. Most molecular methods are appropriate for these specimen types (exception: bone tissue). See individual real-time PCR assay descriptions.

 

Testing information pertaining to specific sources:

Dermal specimens are only acceptable for enterovirus. Clearly indicate "Enterovirus" on test request.

 

Ocular fluids (vitreous and aqueous): viral culture is not recommended due to usually inadequate volumes. PCR testing is recommended. See individual real-time PCR assay descriptions.

 

Genital, synovial fluid, wound swab or tissue (includes pus, drainage, or abscess fluid) specimens are not acceptable for viral culture.

Method Name

Cell Culture

Shell Vial Assay for Herpes Simplex Virus or Cytomegalovirus

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Varies


Advisory Information


Source-based recommendations for testing:

Dermal specimens: Leg, arm, skin, axilla, etc: 

Order LHSV / Herpes Simplex Virus (HSV), Molecular Detection, PCR and/or LVZV / Varicella-Zoster Virus, Molecular, Detection, PCR or LHSVZ / Herpes Simplex Virus (HSV) and Varicella-Zoster Virus (VZV), Molecular Detection, PCR

 

Genital specimens: Cervical, endocervical, genital, labia, penis, perianal, scrotum, vaginal

Order LHSV / Herpes Simplex Virus (HSV), Molecular Detection, PCR

 

Oral specimens: Gum, lip, mouth, palate, tongue, tonsil, parotid, buccal 

Order VRESP / Viral Culture, Respiratory

 

Respiratory specimens: Bronchoalveolar lavage, bronchial wash, lung, lung tissue, nasal swab, nasal wash, sputum, throat swab, tracheal aspirate 

Order VRESP / Viral Culture, Respiratory

 

Infectious agent-based recommendations for testing:

If cytomegalovirus (CMV) is suspected in bone marrow or urine specimens, order LCMV / Cytomegalovirus (CMV), Molecular Detection, PCR 

 

If herpes simplex virus (HSV) is suspected in a neonatal patient (<1 month), order VHSV / Herpes Simplex Virus (HSV), Culture From Neonates

 

State Health Department testing only: specimens will not be accepted at Mayo Medical Laboratories for the following diseases (submit directly to your state health department).

-Measles

-Mumps

-High-risk infectious agents (examples of high-risk infections agents include: Ebola and other causative agents of viral hemorrhagic fever, avian influenza, severe acute respiratory syndrome (SARS), and Middle Eastern respiratory syndrome coronavirus (MERS-CoV)



Shipping Instructions


Specimen must be transported at refrigerate temperature and swab specimens should be sent in viral transport media.

 

Specimens must be received and cultured in the laboratory within 7 days postcollection.

 

Specimens for viral culture should be transported to the laboratory as soon as possible for optimal recovery.



Necessary Information


Specimen source is required.



Specimen Required


Specimen Type: Body fluid

Sources: Pericardial, peritoneal, amniotic

Container/Tube: Sterile container

Specimen Volume: 1 mL

 

Specimen Type: Rectal

Supplies:

Swab, Sterile Polyester (T507)

M5 Media (T484)

Container/Tube: Dacron-tipped swab with plastic handle (T507)

Specimen Volume: Swab

Collection Instructions: Place swab in M5 (T484) or other viral transport media.

 

Specimen Type: Spinal fluid

Container/Tube: Sterile vial

Specimen Volume: 1 mL

 

Specimen Type: Stool

Supplies: Stool Collection Kit, Random (T635)

Container/Tube: Sterile container

Specimen Volume: 5-10 g

 

Specimen Type: Tissue

Sources: Brain, colon, kidney, liver, etc.

Supplies: M5 Media (T484)

Container/Tube: Sterile container with 1 to 2 mL of sterile saline or multi-microbe medium (M5) (T484)

Specimen Volume: Entire collection

 

Specimen Type: Dermal (for enterovirus only)

Supplies:

Swab, Sterile Polyester (T507)

M5 (T484)

Container/Tube: Dacron-tipped swab with plastic handle (T507)

Specimen Volume: Swab

Collection Instructions:

1. Place swab in M5 (T484) or other viral transport media.

2. Clearly label "enterovirus" to ensure proper handling and test setup.


Specimen Minimum Volume

Body Fluid or Spinal Fluid: 1 mL; Stool: 5 g; Urine: 0.5 mL; Tissue Biopsy: 5 mm

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated 7 days

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Gel swab, swab with wood handle, E-swab

Reference Values

Negative

If positive, virus is identified.

Day(s) and Time(s) Performed

Monday through Sunday; Varies

CPT Code Information

87252-Viral culture, non-respiratory

87176-Tissue processing (if appropriate)

87253-Additional testing virus, identification (if appropriate)

87254-Viral smear, shell vial (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
VIRNR Viral Culture, Non Respiratory 6584-7

 

Result ID Test Result Name Result LOINC Value
VIRNR Viral Culture, Non Respiratory 6584-7

Secondary ID

87266

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf).

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.