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Test Code PLHBB Plasma Hemoglobin, Plasma

Reporting Name

Plasma Hemoglobin, P

Useful For

Determining whether hemolysis is occurring such as from:

-Transfusion reaction

-Mechanical fragmentation of red blood cells

-Relative comparison to baseline levels in extracorporeal membrane oxygenation (ECMO) and centrifugal ventricular assist device (cVAD) patients to assess pump disruption

Method Name

Spectrophotometry (SP)

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Plasma EDTA

Specimen Required

Container/Tube: Lavender top (EDTA)
Specimen Volume:
2 mL
Collection Instructions:
 Spin down and transfer plasma to a secondary aliquot tube within 2 hours of draw. IMPORTANT-falsely elevated levels result if left >2 hours due to artifactual post draw RBC lysis.

Forms: If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

Hematopathology/Molecular Oncology Request Form (T241) (

Benign Hematology Test Request Form (T755) (

Specimen Minimum Volume

1.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma EDTA Refrigerated (preferred) 7 days
  Frozen  7 days
  Ambient  4 days

Reject Due To


Mild OK; Gross OK


Mild OK; Gross OK





Reference Values


≥12 months: 0.0-15.2 mg/dL

Reference values have not been established for patients who are <12 months of age.



≥12 months: 0.0-12.4 mg/dL

Reference values have not been established for patients who are <12 months of age.

Day(s) and Time(s) Performed

Monday through Sunday

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
PLHBB Plasma Hemoglobin, P In Process


Result ID Test Result Name Result LOINC Value
31970 Total Hemoglobin 721-1
31971 Oxyhemoglobin In Process

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.