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Test Code LEBV Epstein-Barr Virus (EBV), Molecular Detection, PCR

Reporting Name

Epstein-Barr Virus PCR

Useful For

Rapid qualitative detection of Epstein-Barr virus (EBV) DNA in specimens for laboratory diagnosis of disease due to this virus

Method Name

Real-Time Polymerase Chain Reaction (PCR)/DNA Probe Hybridization

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Varies


Necessary Information


Specimen source is required.



Specimen Required


Supplies: Aliquot Tube, 5 mL (T465)

Specimen Type: Fluid

Sources: Spinal fluid, sterile body fluids (peritoneal fluid/ascites, pericardial fluid, pleural fluid/thoracentesis, amniotic, or ocular

Preferred: 12 x 75-mm screw cap vial (T465)

Acceptable: Sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Do not centrifuge.

 

Supplies: Aliquot Tube, 5 mL (T465)

Specimen Type: Fluid

Sources: Respiratory; bronchial washing, bronchoalveolar lavage, nasopharyngeal aspirate or washing, sputum, or tracheal aspirate

Container/Tube:

Preferred: 12 x 75-mm screw cap vial (T465)

Acceptable: Sterile container

Specimen Volume: 1.5 mL

 

Supplies:

Culturette (BBL Culture Swab) (T092)

M5 Media (T484)

Specimen Type: Swab

Sources: Eye swabs and upper respiratory swabs (nasal, throat)

Container/Tube: BBL CultureSwab (T092) or multimicrobe media (M5) (T484)

Collection Instructions: Place swab into multimicrobe media (M5) (T484) or other viral transport media.

 

Specimen Type: Bone marrow

Container/Tube: Lavender top (EDTA) only

Specimen Volume: 0.5 mL

Additional Information: Clotted specimens will be rejected.

 

Supplies: M5 Media (T484)

Specimen Type: Tissue

Sources: Brain, colon, kidney, liver, lung, etc.

Preferred: Multimicrobe medium (M5) (T484)

Acceptable: Sterile container with 1 to 2 mL of sterile saline

Specimen Volume: Entire collection

Collection Instructions: Submit only fresh tissue.


Specimen Minimum Volume

Body Fluid, Ocular Fluid, Spinal Fluid: 0.3 mL; Respiratory Specimens: 1 mL; Tissue: 2 × 2-mm biopsy

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Calcium alginate-tipped swab, wood swab, or transport swab containing gel; formalin-fixed and paraffin-embedded tissues

Reference Values

Negative

Day(s) and Time(s) Performed

Monday through Friday; 6 a.m.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LEBV Epstein-Barr Virus PCR In Process

 

Result ID Test Result Name Result LOINC Value
SRC67 Specimen Source 31208-2
81239 Epstein-Barr Virus PCR 23858-4

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf)

Secondary ID

81239