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Test Code LAB1318 Shiga Toxin, Molecular Detection, PCR, Feces

Additional Codes

Mayo Test Code: STFRP

Reporting Name

Shiga Toxin PCR, F

Useful For

Sensitive, specific, and rapid detection of the presence of Shiga toxin-producing organisms such as Escherichia coli O157:H7 and Shigella dysenteriae type 1 in stool

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Fecal


Additional Testing Requirements


In some cases, there may be local public health requirements that impact MML clients and require additional testing on specimens with positive results for this test. MML recommends that clients retain an aliquot of each specimen submitted for testing to perform such additional testing, if needed. Alternatively (not preferred), clients who want their specimen returned from MML should call MML as soon as possible, and at the latest within 96 hours of specimen collection, to request that MML return an aliquot of the submitted specimen to them. Clients will be responsible for submitting their specimens to appropriate public health departments.



Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by shiga toxin DNA is unlikely.

 

Submit only 1 of the following specimens:

 

Supplies:

C and S Vial (T058)

Stool container, Small (Random), 4 oz Random (T288)

 

Preferred:

Specimen Type: Preserved stool

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of nonnutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S) (T058)

Specimen Volume: Representative portion of stool

Collection Instructions:

1. Collect fresh stool and submit in container with transport medium.

2. Place stool in preservative within 2 hours of collection.

 

Acceptable:

Specimen Type: Unpreserved stool

Container/Tube: Stool container (T288)

Specimen Volume: Representative portion of stool

Collection Instructions: Collect fresh stool and submit in container.


Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time
Fecal Ambient (preferred) 7 days
  Frozen  7 days
  Refrigerated  7 days

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday through Sunday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
STFRP Shiga Toxin PCR, F In Process

 

Result ID Test Result Name Result LOINC Value
SRC59 Specimen Source 31208-2
56052 Result 33764-2

Reject Due To

Hemolysis

NA

Lipemia

NA

Icterus

NA

Other

Formed stool or stool in gel transport medium; EcoFix preservative; formalin or PVA fixative

Method Name

Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and Fluorescent Resonance Energy Transfer (FRET)

Testing Algorithm

See Laboratory Testing for Infectious Causes of Diarrhea in Special Instructions.

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

Microbiology Test Request Form (T244) (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf)

Gastroenterology and Hepatology Test Request Form (T728) (http://www.mayomedicallaboratories.com/it-mmfiles/gastroenterology-and-hepatology-test-request.pdf)