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Test Code F5DNA Factor V Leiden (R506Q) Mutation, Blood

Reporting Name

Factor V Leiden (R506Q) Mutation, B

Useful For

Factor V Leiden mutation testing should be reserved for patients with clinically suspected thrombophilia and: 1) APC-resistance proven or suspected by a low or borderline APC-resistance ratio, or 2) a family history of factor V Leiden.

Method Name

Direct Mutation Analysis

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Whole blood


Specimen Required


Container/Tube: 

Preferred: Yellow top (ACD)

Acceptable: EDTA or sodium citrate

Specimen Volume: Full tube

Collection Instructions: 

1. Invert several times to mix blood.

2. Send specimen in original tube.

Additional Information: Can be combined with other molecular coagulation tests;

-MTHAC 5,10-Methylenetetrahydrofolate Reductase A1298C, Mutation, Blood

-PTNT Prothrombin G20210A Mutation, Blood

-MTHFR 5,10-Methylenetetrahydrofolate Reductase C677T, Mutation, Blood

-MTHP 5,10-Methylenetetrahydrofolate Reductase C677T and A1298C Mutations, Blood


Specimen Minimum Volume

1 mL blood in a 3-mL ACD tube

Specimen Stability Information

Specimen Type Temperature Time
Whole blood Ambient (preferred) 7 days
  Frozen  14 days
  Refrigerated  14 days

Reject Due To

Hemolysis

Mild OK; Gross OK

Lipemia

Mild OK; Gross OK

Icterus

NA

Other

Green top (heparin) tube or Extracted DNA

Reference Values

Negative

Day(s) and Time(s) Performed

Monday through Friday; 12 p.m.

CPT Code Information

81241-F5 (coagulation factor V) (eg, hereditary hypercoagulability) gene analysis, Leiden variant

 

LOINC Code Information

Test ID Test Order Name Order LOINC Value
F5DNA Factor V Leiden (R506Q) Mutation, B 21668-9

 

Result ID Test Result Name Result LOINC Value
21838 Factor V Leiden (R506Q) Mutation, B 21667-1
21839 F5DNA Interpretation 69049-5
21841 F5DNA Reviewed By 69047-9

Test Classification

This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

Forms

1. Coagulation Patient Information (T675) in Special Instructions

2. New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing form (T576) is available in Special Instructions.

3. If not ordering electronically, complete, print, and send a Coagulation Test Request Form (T753) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/coagulation-test-request-form.pdf)